基于复制带型的海鳟(Salmo trutta morpha trutta)标准核型。

Cytobios Pub Date : 2000-01-01
M Jankun
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引用次数: 0

摘要

利用改良的5-BrdU技术在体内处理Salmo trutta morpha trutta染色体,并在肾脏培养中使用荧光光解Giemsa (FPG)染色法获得了复制带模式。每对染色体根据带型、染色体大小和着丝粒位置进行核型鉴定。提出了海鳟的标准核型。讨论了8号和9号染色体以及11号和12号染色体的复制和限制性内切酶带型的相似性。荧光原位杂交技术用于确定端粒序列的位置。
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Standard karyotype of sea trout (Salmo trutta morpha trutta) based on replication banding patterns.

Replication banding patterns have been obtained from in vivo treatment of Salmo trutta morpha trutta chromosomes using a modification of the 5-BrdU technique and in kidney cultures using the fluorochrome photolysis Giemsa (FPG) staining method. Each chromosome pair was identified in the karyotype based on the banding pattern, chromosome size, and centromere position. The standard karyotype of sea trout has been proposed. Similarities of replication, and restriction enzyme banding patterns of chromosomes 8 and 9, and of chromosomes 11 and 12, are discussed. Fluorescence in situ hybridization was used to determine the location of telomeric sequences.

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