血液透析患者血清中铜、硒、锌、铁的形态分析。

Sbornik lekarsky Pub Date : 2002-01-01

O Mestek, J Komínková, R Koplík, T Zima, M Miskusová, P Stern

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引用次数: 0

摘要

采用等离子体质谱联用技术对血液透析患者血清中微量元素的形态进行了研究。以pH 7.5的0.02 mol/l TRIS-HCl缓冲液为流动相。并与对照组健康人的形态测定结果及总浓度数据进行比较。总浓度的变化仅在硒和锌的情况下观察到。铁以转铁蛋白和谷胱甘肽过氧化物酶的形式存在，铜主要与铜蓝蛋白结合，硒化合物主要为硒蛋白P和谷胱甘肽过氧化物酶。后一种化合物仅在对照组样品中检测到。其他元素的色谱图相似，两组之间没有明显的变化。

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Speciation of Cu, Se, Zn and Fe in blood serum of hemodialysed patients.

The speciation of trace elements in serum samples of hemodialysed patients was investigated using on-line connection of SEC and ICP-MS. The 0.02 mol/l TRIS-HCl buffer of pH 7.5 was used as mobile phase. The results of speciation as well as the total concentration data were compared with those of control group of healthy person. Alterations of total concentration were observed in case of selenium and zinc only. Iron was present in form of transferrin and ferritin, main amount of copper was bound to ceruloplasmin and selenium compounds were identified as selenoproteine P and glutathione peroxidase. The latter compound was detected in samples of control group only. The chromatograms of the other elements were similar and no substantial changes between both investigated groups were observed.

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Sbornik lekarsky

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