Lei Li, Shangwei Li, Zeyi Cao, Zhilan Peng, Ziyan Han
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引用次数: 0
摘要
目的:探讨胰岛素样生长因子(IGFs)在体外早孕人绒毛滋养细胞中的基因表达及其作用。方法:采用半定量逆转录酶聚合酶链式反应(RT-PCR)，以β - actin为内标，检测体外早孕人绒毛滋养细胞中IGFs信使RNA的表达。结果:体外孕早期人绒毛滋养细胞中可检测到IGF-II mRNA、IGF-I R mRNA和IGFBP-3 mRNA的表达。结论:IGFs通过自分泌和/或旁分泌的方式参与细胞滋养细胞的早期侵袭、增殖和分化，以及胎盘的形成和胚胎的发育，发挥重要的调节作用。
[Gene expression of insulin-like growth factors in human villous trophoblast cells of early pregnancy in vitro].
Objective: To investigate the gene expression of insulin-like growth factors(IGFs) in human villous trophoblast cells of early pregnancy in vitro and their action.
Methods: Semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR), with beta-ACTIN as internal standard, was applied to determine the expression of IGFs messenger RNA in human villous trophoblast cells of early pregnancy in vitro.
Results: The expression of IGF-II mRNA, IGF-I R mRNA and IGFBP-3 mRNA was detectable in human villous trophoblast cells of early pregnancy in vitro.
Conclusion: IGFs appear to play important regulation roles in early invasion, proliferation and differentiation of cytotrophoblast, and in the formation of placenta and the development of embryo via autocrine and/or paracrine way.
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