多条带一凝胶法,提高双向电泳的重现性、分辨率和高通量。

Quan Yuan, Jie An, Ding-Gan Liu, Fu-Kun Zhao
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引用次数: 0

摘要

双向聚丙烯酰胺凝胶电泳是蛋白质组学研究中最关键的分离工具之一,一次可揭示数百甚至数千种蛋白质。在本文中,我们报告了一种新的IPG条带的应用,称为多条带一凝胶(MSOG)方法。通过比较相同样品、不同状态样品和相同样品在不同的二维SDS运行系统(大、中尺寸凝胶)中的2-DE模式,我们发现该方法不仅可以提高2-DE模式的重现性和分辨率,而且可以实现高通量和经济格式,有助于自动蛋白质组学研究。
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Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis.

Two-dimensional polyacrylamide gel electrophoresis is one of the most key separation tools which can reveal hundreds or even thousands of proteins at a time in proteomic research. In this paper, we report a new IPG strip application, called multi-strips on one gel (MSOG) method. By comparing the 2-DE patterns of the same sample, the different state samples and the same sample in the different second dimensional SDS running systems (large size and medium size gels), we found this new method can not only improve the reproducibility and resolution power of 2-DE pattern, but also achieve high throughput and economical format which is helpful to automatic proteomic research.

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