[基于MALDI-TOF-PSD质谱的化学辅助碎片法鉴定银染色二维凝胶中的蛋白质斑点]。

Ping Chen, Song Nie, Jin-Yun Xie, Song-Ping Liang, Chong Peng, Gui-Yuan Li
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引用次数: 0

摘要

用o -甲基异脲硫酸氢完成赖氨酸端端胰蛋白酶肽的epsilon-氨基胍,将赖氨酸转化为精氨酸残基,提高了MALDI-MS的检测效率。然后用3-亚砜丙酸nhss -酯化学方法在n端用磺酸基标记色氨酸肽。该衍生物增强了后源衰减(PSD)碎片信号,并产生了仅含y离子的光谱。这促进了从头开始的肽测序,因此它对蛋白质组学研究中明确的蛋白质鉴定做出了重要贡献。该方法已成功应用于鼻咽癌蛋白质组学研究。
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[Identification of protein spots in silver-stained two-dimensional gels based on chemically assisted fragmentation by MALDI-TOF-PSD mass spectrometry].

Guanidination of the epsilon-amino group of lysine-terminated tryptic peptides,accomplished with O-methylisourea hydrogen sulfate, converted lysine into homoarginine residues, improving detection in MALDI-MS. Then tryptic peptides were labeled with sulfonic acid groups at the N-termini using 3-sulfopropionic acid NHS-ester chemistry. The derivatives enhanced post-source decay (PSD) fragmentation signals and produced a spectrum containing only y-ions. This facilitated de novo peptide sequencing, so it is an important contribution to unambiguous protein identification in proteome research. This method was successfully applied in nasopharyngeal carcinoma(NPC) proteome study.

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