烟草花叶病毒增殖过程中游离氨基酸和乙酰化氨基酸的变化

Marvin Karasek
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引用次数: 6

摘要

在对数烟草花叶病毒增殖过程中,测定了对照和侵染烟草植株游离氨基酸和乙酰化氨基酸的变化。在烟草花叶病毒合成的早期(72和144 h),侵染组织中游离氨基酸增加,216 h后下降。在侵染组织和正常植物组织中,乙酰氨基酸的含量不超过0.2 μmol / 100 g。在对数烟草花叶病毒的生物合成过程中,不能证明丝氨酸乙酰化酶(乙酰辅酶a: l-丝氨酸n -乙酰转移酶)的诱导作用。
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Changes in free and acetylated amino acids during tobacco mosaic virus multiplication

Changes in free and acetylated amino acids have been determined in control and infected tobacco plants during logarithmic tobacco mosaic virus multiplication. An increase in free amino acids occurs in infected tissue in the early stages of tobacco mosaic virus synthesis (72 and 144 h) followed by a decrease after 216 h. Acetylamino acids are not present in infected or in normal plant tissue in amounts that exceed 0.2 μmoles per 100 g of plant tissue. The induction of serine acetylase (acetyl-CoA: L-serine N-acetyltransferase) could not be demonstrated during logarithmic tobacco mosaic virus biosynthesis.

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