l-鼠李糖在大肠杆菌中的代谢

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI:10.1016/0926-6569(64)90263-9

Yasuyuki Takagi , Hideo Sawada

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引用次数: 53

摘要

l-鼠李糖异构体酶(l-鼠李糖酮异构酶)已从大肠杆菌提取物中纯化约50倍。该酶似乎是一种SH酶，并催化可逆反应-鼠李糖-鼠李糖糖。添加锰离子使酶活化。在平衡状态下，大约60%的甲基戊糖以l-鼠李糖的形式存在。该酶对l-鼠李糖和l-鼠李糖具有特异性;没有发现其他糖发生反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The metabolism of l-rhamnose in escherichia coli

l-Rhamnose isomearse (l-rhamnose ketol-isomerase) has been purified about 50-fold from extracts of Escherichia coli. THe enzyme appears to be an SH enzyme, and catalyzes the reversible reaction l-rhamnose ⇆ l-rhamnulose. The enzyme was activated by the addition of manganous ions. At equilibrium about 60% of the total methylpentose was present as l-rhamnulose.

The enzyme was specific for l-rhamnose and l-rhamnulose; no other sugars were found to react.

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