小鼠肝脏中可溶性核糖核酸和糖蛋白的生物合成

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI:10.1016/0926-6534(65)90033-3

Hyogo Sinohara, Howard H. Sky-Peck

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引用次数: 28

摘要

腹腔注射[1-14C]葡萄糖胺后，以不同的时间间隔测定小鼠肝脏亚细胞组分的比活性。微粒体微粒的脱氧胆碱可溶性部分是最活跃的。在体内[14C]氨基葡萄糖通过尿苷二磷酸- n -乙酰氨基葡萄糖掺入微粒体片段的过程中，高速上清部分分离的可溶性核糖核酸不被标记。核糖体中的可溶性核糖核酸也未标记。在体外将[14C]葡萄糖胺掺入核糖核酸的尝试也没有成功。讨论了碳水化合物加成多肽链的可能机理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Soluble ribonucleic acid and glycoprotein biosynthesis in the mouse liver

The specific activity of subcellular fractions of liver was determined at varying intervals after the intraperitoneal injection of [1-¹⁴C]glucosamine in mice. The deoxycholate-soluble portion of microsomal particulates was the most active. During the course of [¹⁴C]glucosamine incorporation into microsomal fraction in vivo, by way of uridine diphosphate-N-acetylglucosamine, the soluble ribonucleic acids isolated from the high-speed supernatant fraction were not labeled. Soluble ribonucleic acids from ribosomes were unlabeled also. Attempts to incorporate [¹⁴C]glucosamine into ribonucleic acids in vitro were also unsuccessful. A possible mechanism of carbohydrate addition to polypeptide chains is discussed.

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