电子显微镜下特异性抗体的使用。3用未修饰的抗体定位抗原。

F A PEPE, H FINCK, H HOLTZER
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引用次数: 29

摘要

通过无标记抗体和锇固定在电镜下观察抗体染色。由于抗体沉积在抗原结构上,可见其形态发生了变化。用抗肌凝蛋白、抗h -肌凝蛋白和抗肌动蛋白对甘油化的鸡胸肌进行染色。电子显微镜获得的染色模式与先前荧光显微镜显示的一致。第二种方法用于确认抗体染色。这包括用0.6 M碘化钾,10(-4)M三磷酸腺苷溶液提取肌节未染色部分。由于联合抗原和抗体的不溶性,肌节的染色区域保持完整。
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The use of specific antibody in electron microscopy. III. Localization of antigens by the use of unmodified antibody.

Antibody staining was observed in the electron microscope by means of untagged antibody and osmium fixation. The antibody was visualized as a change in morphology due to its deposition on the antigenic structures. Glycerinated chicken breast muscle was stained with antimyosin, anti-H-meromyosin, and antiactin. The staining patterns obtained by electron microscopy were consistent with those previously demonstrated by fluorescence microscopy. A second method was used for confirmation of antibody staining. This consisted of extraction of unstained portions of the sarcomere with 0.6 M potassium iodide, 10(-4)M adenosine triphosphate solution. Stained regions of the sarcomere remained intact because of insolubility of the combined antigen and antibody.

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