Zhen Ren, Hua Xie, Kathryn A Lagerquist, Allen Burke, Scott Prahl, Kenton W Gregory, Anthony P Furnary
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引用次数: 13
摘要
目的:探讨激光血管焊接中吲哚菁绿(ICG)的最佳浓度和能量辐照度。背景资料:许多研究表明,白蛋白焊料/ICG激光组织焊接可能是外科重建的有效技术。然而,关于激光辅助血管吻合中白蛋白焊点内ICG的最佳激光设置和浓度的报道很少。材料与方法:用50%白蛋白焊料在27.7、56.7、76.9 W/cm(2)的辐照下,端对端用二极管激光焊接猪颈动脉条(n = 120),分别为0.01、0.1、1.0 mM ICG。通过在容器内外插入热电偶来测量温度。研究了拉伸强度和组织学。结果:ICG浓度为56.7 W/cm(2)时,随着ICG浓度的升高,吻合口温度和吻合口强度显著降低(p < 0.05)。组织学研究显示,热损伤仅限于外膜,各组间无明显差异。结论:焊料内ICG浓度是影响血管温度和抗拉强度的最重要因素。强度和最小热损伤之间的最佳平衡可能主要在56.7 W/cm(2)和0.01 mM ICG下实现。
Optimal dye concentration and irradiance for laser-assisted vascular anastomosis.
Objective: This investigation was done in order to find optimal indocyanine green (ICG) concentration and energy irradiance in laser vascular welding.
Background data: Many studies have shown that laser tissue welding with albumin solder/ICG may be an effective technique in surgical reconstruction. However, there are few reports regarding optimal laser settings and concentrations of ICG within the albumin solder in laser-assisted vascular anastomosis.
Materials and methods: Porcine carotid artery strips (n = 120) were welded in end-to-end by diode laser with 50% albumin solder of 0.01, 0.1, and 1.0 mM ICG at irradiance of 27.7, 56.7, and 76.9 W/cm(2), respectively. Temperature was measured by inserting thermocouples outside and inside the vessel. Tensile strength and histology were studied.
Results: Temperature and strength of the anastomosis significantly decreased (all p < 0.05) with increasing ICG concentration at 56.7 W/cm(2). Histological study showed minimal thermal injury limited to adventitia and no appreciable difference between all groups.
Conclusions: ICG concentration within solder is the most important factor affecting both vascular temperature and tensile strength. The optimal balance between strength and minimal thermal injury may be achieved primarily at 56.7 W/cm(2) and 0.01 mM ICG.