Junmin Li , Yuqiao Liu , Ling Zhu , Mingxia Bai , Yanhui Liu , Qin Zhu , Jiaqian Zhao , Dajing Chen
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Continuous glucose metabolism monitoring platform for long-term analysis of tumor cell proliferation and drug response
Glucose metabolism monitoring has emerged as a crucial method for analyzing the metabolic state of tumor cells. Conventional glucose measurement techniques only provide endpoint information and lack the ability for real-time monitoring. Therefore, it is imperative to develop a continuous and long-term monitoring approach to gain insights into cellular behavior and metabolic regulation. We developed a selective permeation outer membrane specifically designed for high glucose concentrations in cell culture, allowing continuous operation in the range of 0–32 mM, significantly exceeding the linear range of typical continuous glucose sensing systems (CGMS). The platform enabled real-time and continuous monitoring of glucose consumption during tumor cell proliferation, demonstrating high linearity, stability, and anti-interference in the cell culture medium. More importantly, by optimizing the electrode design, we achieved accurate and long-term online glucose monitoring for over seven days, double the current capacity. The prepared sensors have enabled continuous monitoring of glucose metabolism parameters in different cell lines, cell numbers and cells treated with different drugs, providing a promising strategy to explore cellular metabolism. The glucose monitoring platform holds tremendous potential in various fields, including drug screening, toxicology, cancer therapy and personalized medicine.
期刊介绍:
The Journal of Electroanalytical Chemistry is the foremost international journal devoted to the interdisciplinary subject of electrochemistry in all its aspects, theoretical as well as applied.
Electrochemistry is a wide ranging area that is in a state of continuous evolution. Rather than compiling a long list of topics covered by the Journal, the editors would like to draw particular attention to the key issues of novelty, topicality and quality. Papers should present new and interesting electrochemical science in a way that is accessible to the reader. The presentation and discussion should be at a level that is consistent with the international status of the Journal. Reports describing the application of well-established techniques to problems that are essentially technical will not be accepted. Similarly, papers that report observations but fail to provide adequate interpretation will be rejected by the Editors. Papers dealing with technical electrochemistry should be submitted to other specialist journals unless the authors can show that their work provides substantially new insights into electrochemical processes.