马链球菌亚种的鉴定与分子特性研究。从肯尼亚和索马里骆驼(单峰骆驼)和骆驼奶中分离的动物流行病。

M Younan, A T S Estoepangestie, M Cengiz, J Alber, A El-Sayed, C Lämmler
{"title":"马链球菌亚种的鉴定与分子特性研究。从肯尼亚和索马里骆驼(单峰骆驼)和骆驼奶中分离的动物流行病。","authors":"M Younan,&nbsp;A T S Estoepangestie,&nbsp;M Cengiz,&nbsp;J Alber,&nbsp;A El-Sayed,&nbsp;C Lämmler","doi":"10.1111/j.1439-0450.2005.00828.x","DOIUrl":null,"url":null,"abstract":"<p><p>Seventeen Streptococcus equi subsp. zooepidemicus strains isolated from camels and camel milk in Kenya and Somalia were identified by their cultural characteristics, by biochemical and serological reactions with the help of commercial identification systems and by molecular studies using a multiplex PCR. The isolates were further characterized by a PCR-mediated detection of size polymorphisms in the 16S-23S rDNA intergenic spacer region and the virulence gene szp and by amplification of the virulence gene cne. These molecular analysis are potentially useful in identifying and characterizing S. equi subsp. zooepidemicus strains of this origin and could possibly be valuable in epidemiological investigations.</p>","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"52 3","pages":"142-6"},"PeriodicalIF":0.0000,"publicationDate":"2005-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.2005.00828.x","citationCount":"20","resultStr":"{\"title\":\"Identification and molecular characterization of Streptococcus equi subsp. zooepidemicus isolated from camels (Camelus dromedarius) and camel milk in Kenya and Somalia.\",\"authors\":\"M Younan,&nbsp;A T S Estoepangestie,&nbsp;M Cengiz,&nbsp;J Alber,&nbsp;A El-Sayed,&nbsp;C Lämmler\",\"doi\":\"10.1111/j.1439-0450.2005.00828.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Seventeen Streptococcus equi subsp. zooepidemicus strains isolated from camels and camel milk in Kenya and Somalia were identified by their cultural characteristics, by biochemical and serological reactions with the help of commercial identification systems and by molecular studies using a multiplex PCR. The isolates were further characterized by a PCR-mediated detection of size polymorphisms in the 16S-23S rDNA intergenic spacer region and the virulence gene szp and by amplification of the virulence gene cne. These molecular analysis are potentially useful in identifying and characterizing S. equi subsp. zooepidemicus strains of this origin and could possibly be valuable in epidemiological investigations.</p>\",\"PeriodicalId\":17659,\"journal\":{\"name\":\"Journal of veterinary medicine. B, Infectious diseases and veterinary public health\",\"volume\":\"52 3\",\"pages\":\"142-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1439-0450.2005.00828.x\",\"citationCount\":\"20\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of veterinary medicine. B, Infectious diseases and veterinary public health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/j.1439-0450.2005.00828.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1439-0450.2005.00828.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 20

摘要

17马链球菌亚种从肯尼亚和索马里的骆驼和骆驼奶中分离出的动物流行病菌株通过其培养特征、在商业鉴定系统的帮助下通过生化和血清学反应以及使用多重聚合酶链反应的分子研究进行了鉴定。通过pcr介导的16S-23S rDNA基因间隔区和毒力基因szp的大小多态性检测以及毒力基因cne的扩增,进一步对分离株进行了鉴定。这些分子分析在鉴定和鉴定马链球菌亚种方面具有潜在的价值。这种来源的动物流行毒株,可能在流行病学调查中有价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Identification and molecular characterization of Streptococcus equi subsp. zooepidemicus isolated from camels (Camelus dromedarius) and camel milk in Kenya and Somalia.

Seventeen Streptococcus equi subsp. zooepidemicus strains isolated from camels and camel milk in Kenya and Somalia were identified by their cultural characteristics, by biochemical and serological reactions with the help of commercial identification systems and by molecular studies using a multiplex PCR. The isolates were further characterized by a PCR-mediated detection of size polymorphisms in the 16S-23S rDNA intergenic spacer region and the virulence gene szp and by amplification of the virulence gene cne. These molecular analysis are potentially useful in identifying and characterizing S. equi subsp. zooepidemicus strains of this origin and could possibly be valuable in epidemiological investigations.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Keratoconjunctivitis and periorbital papillomatosis associated with heavy periorbital infestation by the tail louse Haematopinus quadripertusus in heifers. Comparative study of PCR as a direct assay and ELISA and AGID as indirect assays for the detection of bovine leukaemia virus. Histopathological and immunohistochemical study of lambs experimentally infected with Fasciola hepatica and Schistosoma bovis. Use of polymerase chain reaction to identify Brucella abortus strain RB51 among Brucella field isolates from cattle in Italy. Use of monoclonal antibodies in blocking ELISA detection of transmissible gastroenteritis virus in faeces of piglets.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1