C Purushotham Reddy, K Ramakrishna, Kmv Narayana Rao
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引用次数: 0
摘要
硼替佐米(BT)是一种可逆的蛋白酶体抑制剂，用于治疗血液病癌症。为了研究BT的降解行为，根据ICH指南Q1A (R2)，对其进行了酸性、碱性、中性、光解、氧化和热降解条件的研究。在Waters XBridge C18色谱柱(150 mm × 4.6 mm × 3.5µm)上，以甲酸铵和乙腈为流动相，建立了梯度高效液相色谱法分离不同降解条件下形成的所有降解产物。在不同的应力条件下，共形成了6种降解产物，并利用高效液相色谱法结合电喷雾电离串联质谱法对其进行了分离鉴定和表征。
Separation, identification and characterization of stress degradation products of bortezomib using HPLC and LC-MS.
Bortezomib (BT) is a reversible inhibitor of proteasome which is used in the treatment of hematological cancers. To study the degradation behavior, BT was subjected to acidic, basic, neutral, photolytic, oxidative and thermal degradation conditions as per ICH guideline Q1A (R2). A gradient HPLC method has been developed for separating all the degradation products formed under various degradation conditions on Waters XBridge C18 column (150 mm × 4.6 mm × 3.5 µm) using the mobile phase composed of ammonium formate and acetonitrile. A total of six degradation products were formed in various stress conditions and these were separated identified, and characterized using high performance liquid chromatography in combination with electrospray ionization tandem mass spectrometric studies.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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