噬菌体衣壳上的显示文库。

Gabriella Garufi, Olga Minenkova, Carla Lo Passo, Ida Pernice, Franco Felici
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引用次数: 32

摘要

噬菌体展示是一项成熟的技术,在过去的15年里,已经成功地应用于旨在破译生物过程和/或在几种不同应用中分离具有实用价值的分子的项目。噬菌体λ (Bacteriophage lambda)是几十年来广泛使用的分子克隆和表达工具,也被用来开发在其衣壳上展示文库的载体。在过去的几年中,lambda显示方法一直提供新的迷人的技术应用前景,如结构域作图、抗原发现和蛋白质相互作用研究,或者更普遍地说,在功能基因组学中。
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Display libraries on bacteriophage lambda capsid.

Phage display is an established technology that has been successfully applied, in the last fifteen years, to projects aimed at deciphering biological processes and/or at the isolation of molecules of practical value in several diverse applications. Bacteriophage lambda, representing a molecular cloning and expression tool widely utilized since decades, has also been exploited to develop vectors for the display of libraries on its capsid. In the last few years, lambda display approach has been consistently offering new enthralling perspectives of technological application, such as domain mapping, antigen discovery, and protein interaction studies or, more generally, in functional genomics.

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