cms水稻线粒体基因组的RFLP分析

HUANG Wei , WANG Li , YI Ping , TAN Xue-Lin , ZHANG Xue-Mei , ZHANG Zai-Jun , LI Yang-Sheng , ZHU Ying-Guo
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引用次数: 8

摘要

采用限制性内切片段长度多态性(RFLP)对水稻细胞质雄性不育(CMS)的线粒体基因组进行了分析。9种CMS水稻的不育系(A)和维持系(B)的线粒体基因组存在差异;线粒体基因组在A和B之间的许多功能基因区域也存在差异。即使是具有相同核酸背景的物质,其mtDNA也存在差异。这为细胞质异质性和CMS机制研究提供了分子证据。
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RFLP Analysis for Mitochondrial Genome of CMS-Rice

Restriction fragment length polymorphism (RFLP) was used to analyze mitochondrial (mt) genome of cytoplasmic male sterility (CMS) rice. Differences were observed among mitochondrial genomes of the sterile line (A) and maintain line (B) of nine types of CMS rice; Mitochondrial genomic differences were also detected between A and B in many functional gene regions. Even the materials with the same nucleic background have differences in their mtDNA. This provides molecular evidence for the cytoplasmic heterogeneity and the CMS mechanism research.

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