S O'mahony, M Longfellow, M J McMahon, A T Axon, P Quirke
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引用次数: 9
摘要
目的:研究胰腺癌及其他胆道肿瘤所致胆道狭窄患者的胆汁中是否可检测到c-Ki-ras突变,以期将胆汁c-Ki-ras突变作为胰腺癌的无创诊断标志物。方法:收集89例患者的胆汁,其中对照组47例(包括胆管结石或良性狭窄患者),胰腺癌患者20例,胆管癌患者11例,壶腹癌患者5例,转移性胆管梗阻患者6例),行内镜逆行胆管造影。从胆汁中提取DNA,用PCR和限制性内切酶法检测c-Ki-ras密码子12突变。结果:20例胰腺癌患者中有10例(50%)检测到c- ki -ras突变,11例胆管癌患者中有1例(9%)检测到c- ki -ras突变,6例转移性胆道梗阻(原发肿瘤:结肠癌和前列腺)患者中有2例(33%)检测到c- ki -ras突变。在对照组和壶腹癌患者中未检测到C-Ki-ras突变。结论:该试验的灵敏度低至50%,不推荐临床使用,但经过改进,有可能作为胰腺癌的诊断标志物。
Detection of c-Ki-ras mutations in bile samples from patients with pancreatic and biliary cancers.
Aim-To determine whether c-Ki-ras mutations can be detected in bile from patients with biliary strictures caused by pancreatic cancer and other biliary tumours, with a view to developing bile c-Ki-ras mutations as a non-invasive diagnostic marker of pancreatic cancer.Methods-Bile was collected from 89 subjects (47 controls (including patients with bile duct stones or benign stricture), 20 patients with pancreatic cancer, 11 with cholangiocarcinoma, five with ampullary cancer, and six with metastatic biliary obstruction) referred for endoscopic retrograde cholangiopancreatography. DNA was extracted from bile and c-Ki-ras codon 12 mutations were detected using PCR and a restriction enzyme digestion method.Results-c-Ki-ras mutations were detected in 10 (50%) of 20 patients with pancreatic cancer, in one (9%) of 11 with cholangiocarcinoma, and in two (33%) of six patients with metastatic biliary obstruction (primary tumours: colon and prostate). C-Ki-ras mutations were not detected in the controls and patients with ampullary cancer.Conclusions-The sensitivity of this test is too low at 50% to recommend its use clinically, but with refinement has potential as a diagnostic marker for pancreatic cancer.