Mark Prescott, Jion M Battad, Pascal G Wilmann, Jamie Rossjohn, Rodney J Devenish
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引用次数: 15
摘要
绿色荧光蛋白(GFP)是一项强大技术的基础，它彻底改变了生命科学家在活细胞中进行实验的方式。通过现有蛋白质的开发和全蛋白发色团(APC)家族新成员的发现，该技术不断发展和改进。本文综述了该技术的最新进展，特别关注具有光学特性的apc，相对于来自Aequorea victoria GFP的变体，apc具有显着的红移。
Recent advances in all-protein chromophore technology.
The green fluorescent protein (GFP) is the foundation of a powerful technology that has revolutionized the way in which the life scientist carries out experiments in the living cell. The technology is continually evolving and improving through the development of existing proteins and discovery of new members of the all-protein chromophore (APC) family. This review gives an overview of the more recent advances in the technology with a particular focus on APCs having optical properties that are significantly red-shifted relative to those variants derived from Aequorea victoria GFP.
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