一种测定青霉素结合蛋白的方法。

Michael J Pucci, Thomas J Dougherty
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引用次数: 3

摘要

组装细菌细胞壁的关键酶也是β -内酰胺类抗生素的目标。标记青霉素与这些蛋白的共价结合已被用于药物发现、抗生素作用机制和耐药性以及细胞壁生理学的许多研究中。描述了标记和测量两种典型生物中青霉素结合蛋白的方法,革兰氏阴性(大肠杆菌)和革兰氏阳性(金黄色葡萄球菌)。讨论的方法包括在完整细胞(体内测量)和分离细胞膜中鉴定青霉素结合蛋白。
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A method to assay penicillin-binding proteins.

Key enzymes that assemble the bacterial cell wall are also the target of the Beta-lactam class of antibiotics. The covalent binding of labeled penicillin to these proteins has been used in numerous studies in drug discovery, antibiotic mechanisms of action and resistance, and cell wall physiology. Methods to label and measure penicillin binding proteins in two prototypical organisms, a Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus), are described. The methods discussed include identifying penicillin-binding proteins in both intact cells (in vivo measurements) and isolated cell membranes.

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