快速可逆RNA光交联的研究进展。

Yoshinaga Yoshimura, Tomoko Ohtake, Hajime Okada, Kenzo Fujimoto
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引用次数: 3

摘要

我们描述了一种新的可逆的RNA链间光交联反应。在该体系中,含有3-氰酰咔唑核苷(CNV)K的修饰寡脱氧核苷酸(ODN)在366nm光照射下与互补模板RNA的嘧啶残基反应1s,得到交联产物,产率为94%。
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Development of a rapid and reversible photocrosslinking of RNA.

We describe a novel reversible interstrand photocrosslinking reaction of RNA. In this system, a modified oligodeoxynucleotide (ODN) containing 3-cyanovinylcarbazole nucleoside ((CNV)K) reacts by photoirradiation at 366 nm for 1 s with pyrimidine residue of a complementary template RNA to yield a crosslinked product in 94% yield.

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