硅烷化芘修饰DNA荧光发射机理的研究。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp016

Tomohisa Moriguchi, Masayoshi Hattori, Tohru Sekiguchi, Kazuo Shinozuka

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引用次数: 2

摘要

在5′端含有硅烷化芘衍生物的修饰DNA表现出明显的双相构象的辨别能力，即在有互补DNA的情况下，修饰DNA表现出很强的荧光发射，而在没有互补DNA的情况下，修饰DNA的荧光发射很弱。发现双链的荧光发射强度比单链增加了约10倍。在本文中，我们试图揭示这种识别能力的硅化芘修饰DNA的详细机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Investigation of the mechanism of the fluorescent emission of the silylated pyrene modified DNA.

Modified DNA containing silylated pyrene derivative at the 5'-terminus showed the obvious discrimination ability of the duplex formation, that the modified DNA showed the strong fluorescence emission in the presence of the complementary DNA, in spite of the very weak emission in the absence of the complementary DNA. The intensity of the fluorescence emission of the double strand found to increase about 10 times compared with that of the single strand. In this paper, we tried to disclose the detailed mechanism of such a discrimination ability of the silylated pyrene-modified DNA.

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Nucleic acids symposium series (2004)

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