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引用次数: 0
摘要
合成了含有2- n -叔丁基氨基氧化腺苷(1)的寡脱氧核苷酸(ODN, 3)，并研究了3及其双链物的EPR谱。在3 ~ 5-8之间的双相化合物的EPR光谱中的h(+)/h(0)值与Tm值有很好的相关性。我们还合成了含有2的odn4，它通过一个连接体有一个环氨基氧基，与odn3比较。在4 ~ 5-8之间的双相化合物的EPR光谱中的h(+)/h(0)值与Tm值不相关。这些结果表明，1具有监测核碱基运动的潜力。
Evaluation of mobility of 2-N-tert-butylaminoxyladenosine incorporated into oligodeoxynucleotides.
We synthesized oligodeoxynucleotide (ODN, 3) containing 2-N-tert-butylaminoxyladenosine (1) and studied EPR spectra of 3 and its duplexes. The h(+)/h(0) values in the EPR spectra of duplexes between 3 and 5-8 well correlated with Tm values. We also synthesized ODN 4 containing 2, which has a cyclic aminoxyl via a linker, to compare with ODN 3. The h(+)/h(0) values in the EPR spectra of duplexes between 4 and 5-8 did not correlate with Tm values. These results indicate that 1 has a potential to monitor of motion of the nucleobase.
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