{"title":"[一步逆转录-聚合酶链反应法检测腮腺炎病毒疏水酶基因的建立]。","authors":"Xin Chang, Jian-Hui Zhou, Shuang Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).</p><p><strong>Methods: </strong>To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.</p><p><strong>Results: </strong>At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.</p><p><strong>Conclusions: </strong>The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.</p>","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Establishment of one-step reverse transcription-polymerase chain reaction method for mumps virus hydrophobin gene detection].\",\"authors\":\"Xin Chang, Jian-Hui Zhou, Shuang Wang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).</p><p><strong>Methods: </strong>To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.</p><p><strong>Results: </strong>At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.</p><p><strong>Conclusions: </strong>The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.</p>\",\"PeriodicalId\":56402,\"journal\":{\"name\":\"中国疫苗和免疫\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国疫苗和免疫\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国疫苗和免疫","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Establishment of one-step reverse transcription-polymerase chain reaction method for mumps virus hydrophobin gene detection].
Objective: To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).
Methods: To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.
Results: At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.
Conclusions: The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.
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