印度金奈海滩产壳聚糖细菌的分离与鉴定。

Q2 Biochemistry, Genetics and Molecular Biology Enzyme Research Pub Date : 2012-01-01 Epub Date: 2012-08-05 DOI:10.1155/2012/421683
Kuldeep Kaur, Vikrant Dattajirao, Vikas Shrivastava, Uma Bhardwaj
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引用次数: 46

摘要

壳聚糖是由几丁质去乙酰化酶(一种水解几丁质中n -乙酰氨基葡萄糖胺的酶)产生的几丁质去乙酰化产物。壳聚糖是一种天然高分子材料,在生物技术和生物医药工业中具有巨大的应用潜力。商业上,它是通过苛刻的热化学过程从几丁质中生产出来的,该过程具有多步骤化学过程的大部分缺点。它对环境不安全,不易控制,导致产品种类繁多。利用甲壳素酶去乙酰化的替代或补充程序可能被采用,特别是当需要一个控制和定义明确的过程时。在这项研究中,从印度金奈不同海滩收集的土壤样品中分离出20株细菌。在这20株细菌中,只有2株(S3、S14)是几丁质的有效降解菌,它们也是几丁质脱乙酰酶的良好产生菌,从而释放壳聚糖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Isolation and characterization of chitosan-producing bacteria from beaches of chennai, India.

Chitosan is a deacetylated product of chitin produced by chitin deacetylase, an enzyme that hydrolyses acetamido groups of N-acetylglucosamine in chitin. Chitosan is a natural polymer that has great potential in biotechnology and in the biomedical and pharmaceutical industries. Commercially, it is produced from chitin via a harsh thermochemical process that shares most of the disadvantages of a multistep chemical procedure. It is environmentally unsafe and not easily controlled, leading to a broad and heterogeneous range of products. An alternative or complementary procedure exploiting the enzymatic deacetylation of chitin could potentially be employed, especially when a controlled and well-defined process is required. In this study, 20 strains of bacteria were isolated from soil samples collected from different beaches of Chennai, India. Of these 20 bacterial strains, only 2 strains (S3, S14) are potent degrader of chitin and they are also a good producer of the enzyme chitin deacetylase so as to release chitosan.

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Enzyme Research
Enzyme Research Biochemistry, Genetics and Molecular Biology-Biochemistry
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