Vasco Elbrecht, Lisa Poettker, Uwe John, Florian Leese
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引用次数: 30
摘要
利用已知的Pteronarcys princeps Banks, 1907 (Pteronarcyidae)序列，采用454和Sanger联合测序方法对perlid stonefly Dinocras cephalotes (Curtis, 1827)的线粒体全基因组进行测序，以确定同源454 reads。基因组全长15666 bp，包括13个蛋白质编码基因、2个核糖体RNA基因、22个转移RNA基因和1个控制区。基因顺序类似于基生节肢动物。基因组碱基组成为A(33.5%)、T(29.0%)、C(24.4%)和G(13.1%)。这是第二次发表的翼翅目有丝分裂基因组，对今后的系统发育分析具有重要意义。
The complete mitochondrial genome of the stonefly Dinocras cephalotes (Plecoptera, Perlidae).
The complete mitochondrial genome of the perlid stonefly Dinocras cephalotes (Curtis, 1827) was sequenced using a combined 454 and Sanger sequencing approach using the known sequence of Pteronarcys princeps Banks, 1907 (Pteronarcyidae), to identify homologous 454 reads. The genome is 15,666 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of basal arthropods. The base composition of the genome is A (33.5%), T (29.0%), C (24.4%) and G (13.1%). This is the second published mitogenome for the order Plecoptera and will be useful in future phylogenetic analysis.
期刊介绍：
Previously published under the title DNA Sequence (Vols 1-19.3), Mitochondrial DNA accepts original high-quality reports based on mapping, sequencing and analysis of mitochondrial DNA and RNA. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, medical genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The editorial board will also consider manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences.
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