猪慢性心肌梗死闭合性胸模型的建立:磁共振成像和病理评价。

ISRN cardiology Pub Date : 2013-10-27 eCollection Date: 2013-01-01 DOI:10.1155/2013/781762
Verónica Crisóstomo, Juan Maestre, Manuel Maynar, Fei Sun, Claudia Báez-Díaz, Jesús Usón, Francisco M Sánchez-Margallo
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引用次数: 24

摘要

我们的目的是利用冠状动脉内乙醇建立一种易于诱导、可重复、低死亡率的猪慢性心肌梗死临床相关闭胸模型,并利用MRI和病理学表征其演变。我们将3-4 mL的100%乙醇注射到麻醉猪的lad中期。心功能和梗死面积通过MRI连续评估。分别于第7、30和90天(每个时间点n = 5)对猪实施安乐死。术后MRI显示收缩性受损,射血分数下降,从53.8%±6.32%降至43.79%±7.72% (P = 0.001)。随访期间,这些数值均低于基线值(46.54%±11.12%,44.48%±7.77%,40.48%±6.40%)。, p < 0.05)。所有动物均可见进行性重塑。梗死心肌在前30天下降(由18.09%±7.26%降至9.9%±5.68%),后稳定(10.2%±4.21%)。病理显示胶原蛋白含量和纤维组织随着时间的推移而增加,心内膜细胞边缘保存完好。综上所述,猪冠状动脉内注射乙醇会导致梗死。随着时间的推移,心功能和心肌变薄的持续妥协表明,该模型可能对心衰治疗方法的临床前评估和培训有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Development of a closed chest model of chronic myocardial infarction in Swine: magnetic resonance imaging and pathological evaluation.

Our aim was to develop an easy-to-induce, reproducible, and low mortality clinically relevant closed-chest model of chronic myocardial infarction in swine using intracoronary ethanol and characterize its evolution using MRI and pathology. We injected 3-4 mL of 100% ethanol into the mid-LAD of anesthetized swine. Heart function and infarct size were assessed serially using MRI. Pigs were euthanized on days 7, 30, and 90 (n = 5 at each timepoint). Postoperative MRI revealed compromised contractility and decreased ejection fraction, from 53.8% ± 6.32% to 43.79% ± 7.72% (P = 0.001). These values remained lower than baseline thorough the followup (46.54% ± 11.12%, 44.48% ± 7.77%, and 40.48% ± 6.40%, resp., P < 0.05). Progressive remodeling was seen in all animals. Infarcted myocardium decreased on the first 30 days (from 18.09% ± 7.26% to 9.9% ± 5.68%) and then stabilized (10.2% ± 4.21%). Pathology revealed increasing collagen content and fibrous organization over time, with a rim of preserved endocardial cells. In conclusion, intracoronary ethanol administration in swine consistently results in infarction. The sustained compromise in heart function and myocardial thinning over time indicate that the model may be useful for the preclinical evaluation of and training in therapeutic approaches to heart failure.

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