牛卵母细胞单株培养的体外成熟系统。

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES Japanese Journal of Veterinary Research Pub Date : 2013-11-01

Masashi Nagano, Sung-Sik Kang, Keisuke Koyama, Weiping Huang, Yojiro Yanagawa, Yoshiyuki Takahashi

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引用次数: 0

摘要

为了对牛卵母细胞个体体外成熟(IVM)培养进行初步研究，采用了多孔(MW)平板。观察了50微滴中成组培养和10微滴中单独培养的IVM卵母细胞的成熟、受精和囊胚发育情况。各实验组的成熟率相似。MW和50微滴的正常受精率相似，但10微滴的受精率较低(p < 0.01)。10微滴的囊胚率明显低于50微滴(p = 0.19)和MW (p = 0.15)。这些结果表明，使用MW的IVM系统支持牛卵母细胞获得与传统群体IVM培养相同的发育能力。

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In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate.

As a preliminary study for the development of individual in vitro maturation (IVM) culture of bovine oocytes, a multi-well (MW) plate was used. Maturation, fertilization and development to blastocysts were examined and compared with those of IVM oocytes cultured in 50-microl droplets in groups and in 10-microl droplets individually. The maturation rates were similar in all experimental groups. Normal fertilization rates in MW and 50-microl droplets were similar, but lower in 10-microl droplets (p < 0.01). The blastocyst rate in 10-microl droplets tended to be lower than those in MW (p = 0.15) and 50-microl droplets (p = 0.19). These results indicate that an IVM system using MW supports the acquisition of developmental competence by bovine oocytes the same as conventional group IVM culture.

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来源期刊

Japanese Journal of Veterinary Research 农林科学-兽医学

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Japanese Journal of Veterinary Research (JJVR) quarterly publishes peer-reviewed articles on all aspects of veterinary science. JJVR was originally published as a “University Journal” of veterinary science at Hokkaido University from more than 60 years ago. Currently, JJVR, is Japan’s leading scientific veterinary journal, and provides valuable information for the development of veterinary science by welcoming contributions from researchers worldwide. JJVR offers online submission for Regular Papers, Short Communications, and Review Articles that are unpublished and not being considered for publication elsewhere. Research areas include: Anatomy, Physiology, Biochemistry, Pharmacology, Microbiology, Infectious diseases, Parasitology, Laboratory Animal Science and Medicine, Internal Medicine, Surgery, Pathology, Theriogenology, Molecular Medicine, Public Health, Radiation Biology, Toxicology, Wildlife Biology and Medicine, Veterinary Hygiene, The other fields related to veterinary science.