放线菌生产生物活性化合物及其抗氧化性能。

Biotechnology Research International Pub Date : 2014-01-01 Epub Date: 2014-03-26 DOI:10.1155/2014/217030
Avilala Janardhan, Arthala Praveen Kumar, Buddolla Viswanath, D V R Saigopal, Golla Narasimha
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引用次数: 95

摘要

从印度安得拉邦Nellore地区的红树林土壤中分离到一株放线菌,并对其产活性化合物的能力进行了筛选。培养、形态学和生化特性及16S rRNA测序结果表明,分离菌株为白色诺卡藻。用柱层析法纯化了该菌株产生的生物活性化合物。对分离的化合物(馏分)的体外抗氧化能力进行了估计,馏分F2显示出与标准抗坏血酸非常接近的值。用高效液相色谱法对柱层析得到的电位组分进行进一步纯化,然后用FTIR、NMR和质谱对纯化后的电位组分F2进行化学结构分析。通过光谱数据预测,分离得到的化合物结构为“(Z)-1-((1-羟基五苯-2,4-二烯-1-基)氧)蒽-9,10-二酮”。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Production of bioactive compounds by actinomycetes and their antioxidant properties.

An actinomycete was isolated from mangrove soil collected from Nellore region of Andhra Pradesh, India, and screened for its ability to produce bioactive compounds. The cultural, morphological, and biochemical characters and 16S rRNA sequencing suggest that the isolated strain is Nocardiopsis alba. The bioactive compounds produced by this strain were purified by column chromatography. The in vitro antioxidant capacity of the isolated compounds (fractions) was estimated and fraction F2 showed very near values to the standard ascorbic acid. The potential fraction obtained by column chromatography was subjected to HPLC for further purification, then this purified fraction F2 was examined by FTIR, NMR, and mass spectroscopy to elucidate its chemical structure. By spectral data, the structure of the isolated compound was predicted as "(Z)-1-((1-hydroxypenta-2,4-dien-1-yl)oxy)anthracene-9,10-dione."

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