研究糖尿病肾病的小鼠模型

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2015-06-01 DOI:10.1002/9780470942390.mo140192

Bryna S.M. Chow, Terri J. Allen

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引用次数: 20

摘要

糖尿病肾病(DN)是一个用来描述由糖尿病引起的肾脏损害的术语。DN是世界范围内终末期肾脏疾病的主要病因之一，因此迫切需要合适的动物模型来研究DN的发病机制和制定治疗策略。迄今为止，大多数实验都是在小鼠模型中进行的，而不是在其他物种中进行，原因包括成本较低，易于处理，易于操纵小鼠基因组以产生转基因和基因敲除动物。本单元提供详细的见解和技术知识，建立最广泛使用的DN模型之一，链脲佐菌素(STZ)诱导的模型。该模型已被广泛用于研究糖尿病肾损伤的机制。讨论了STZ遗传模型的优点和局限性以及其他遗传模型的可用性。©2015 by John Wiley &儿子,Inc。

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Mouse Models for Studying Diabetic Nephropathy

Diabetic nephropathy (DN) is a term used to describe kidney damage cause by diabetes. With DN as one of the leading causes of end-stage renal disease worldwide, there is a strong need for appropriate animal models to study DN pathogenesis and develop therapeutic strategies. To date, most experiments are carried out in mouse models as opposed to other species for several reasons including lower cost, ease of handling, and easy manipulation of the mouse genome to generate transgenic and knockout animals. This unit provides detailed insights and technical knowledge in setting up one of the most widely used models of DN, the streptozotocin (STZ)-induced model. This model has been extensively exploited to study the mechanism of diabetic renal injury. The advantages and limitations of the STZ model and the availability of other genetic models of DN are also discussed. © 2015 by John Wiley & Sons, Inc.

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.