M M Khalisov, A V Ankudinov, V A Penniyaynen, D Dobrota, Boris V Krylov
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Application of atomic force microscopy for investigation of Na(+),K(+)-ATPase signal-transducing function.
The Young's modulus of 10-12-day-old chick embryos' sensory neurons cultivated in dissociated cell culture was measured using a PeakForce Quantitative Nanomechanical Mapping atomic force microscopy. The native cells were tested in control experiments and after application of ouabain. At low "endogenous" concentration of 10⁻¹⁰ M, ouabain tended to increase the rigidity of sensory neurons. We hypothesize that this trend resulted from activation of Na⁺,K⁺-ATPase signal-transducing function.
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