用一些计算和实验方法研究曲霉属植物菊粉酶的过分子组织

Biofizika Pub Date : 2015-07-01
M G Holyavka, V G Artyukhov, S M Makin
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引用次数: 0

摘要

设计了无花果曲霉菊粉酶二聚体的计算机模型。实验研究了黑曲霉菊粉酶的过分子结构。讨论了各种菊粉酶在其功能活性表现中的作用问题。结果表明,在菊粉酶二聚化过程中,当酶的单体形式之间形成接触时,非极性氨基酸残基起着关键作用。
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[Investigation of Inulinase Permolecular Organization from Producers of the Genus Aspergillus by Means of Some Computing and Experimental Methods].

The computer model for a dimer of inulinase from Aspergillus ficuum is designed. The permolecular organization of inulinase from Aspergillus niger is experimentally investigated. The question about the role of various inulinase forms in manifestation of their functional activity is discussed. It is shown, that in the process of inulinase dimerization when contacts between monomeric forms of the enzyme are formed, a key role belongs to the nonpolar amino acid residues.

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