电子显微镜的发展改变了膜蛋白结构生物学的面貌。

Q3 Biochemistry, Genetics and Molecular Biology Molecular Membrane Biology Pub Date : 2016-03-01 Epub Date: 2016-09-09 DOI:10.1080/09687688.2016.1221533

Shaun Rawson, Simon Davies, Jonathan D Lippiat, Stephen P Muench

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引用次数: 0

摘要

膜蛋白在生物学中无处不在，是治疗开发的关键目标。尽管如此，我们对膜蛋白结构的了解仍落后于对可溶性膜蛋白的了解。这篇综述概述了这一重要领域，特别关注电子显微镜（EM）最近的复苏及其在膜蛋白结构研究中发挥的越来越重要的作用，并通过几个案例研究来说明这一点。此外，我们还探讨了在结构确定方面仍然存在的一些挑战，以及为增进我们对这些神秘蛋白质的了解而正在采取的措施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The changing landscape of membrane protein structural biology through developments in electron microscopy.

Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy (EM) and the increasing role it has to play in the structural studies of membrane proteins, and illustrating this through several case studies. In addition, we examine some of the challenges remaining in structural determination, and what steps are underway to enhance our knowledge of these enigmatic proteins.

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来源期刊

Molecular Membrane Biology 生物-生化与分子生物学

CiteScore

4.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Cessation. Molecular Membrane Biology provides a forum for high quality research that serves to advance knowledge in molecular aspects of biological membrane structure and function. The journal welcomes submissions of original research papers and reviews in the following areas: • Membrane receptors and signalling • Membrane transporters, pores and channels • Synthesis and structure of membrane proteins • Membrane translocation and targeting • Lipid organisation and asymmetry • Model membranes • Membrane trafficking • Cytoskeletal and extracellular membrane interactions • Cell adhesion and intercellular interactions • Molecular dynamics and molecular modelling of membranes. • Antimicrobial peptides.