单点单分子FRAP法测定核膜上膜蛋白分布。

Q3 Biochemistry, Genetics and Molecular Biology Current Protocols in Cell Biology Pub Date : 2017-09-01 DOI:10.1002/cpcb.27

Krishna C Mudumbi, Weidong Yang

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Determination of Membrane Protein Distribution on the Nuclear Envelope by Single-Point Single-Molecule FRAP.

Nuclear envelope transmembrane proteins (NETs) are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM) in eukaryotic cells. The abnormal distribution of NETs has been associated with many human diseases. However, quantitative determination of the spatial distribution and translocation dynamics of NETs on the ONM and INM is still very limited in currently existing approaches. Here we demonstrate a single-point single-molecule fluorescence recovery after photobleaching (FRAP) microscopy technique that enables quick determination of distribution and translocation rates for NETs in vivo. © 2017 by John Wiley & Sons, Inc.

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Current Protocols in Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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期刊介绍： Developed by leading scientists in the field, Current Protocols in Cell Biology is an essential reference for researchers who study the relationship between specific molecules and genes and their location, function and structure at the cellular level. Updated every three months in all formats, CPCB is constantly evolving to keep pace with the very latest discoveries and developments.

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