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{"title":"利用网络BLAST搜索在氨基酸序列中寻找同源物","authors":"Istvan Ladunga","doi":"10.1002/cpbi.34","DOIUrl":null,"url":null,"abstract":"<p>BLAST, the Basic Local Alignment Search Tool, is used more frequently than any other biosequence database search program. We show how to run searches on the Web, and demonstrate how to increase performance by fine-tuning arguments for a specific research project. We offer guidance for interpreting results, statistical significance and biological relevance issues, and suggest complementary analyses. This unit covers both protein-to-protein (<i>blastp</i>) searches and translated searches (<i>blastx, tblastn, tfastx)</i>. <i>blastx</i> conceptually translates the query sequence and <i>tblastn</i> translates all nucleotide sequences in a database, while <i>tblastx</i> translates both the query and the database sequences into amino acid sequences. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":10958,"journal":{"name":"Current protocols in bioinformatics","volume":"59 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpbi.34","citationCount":"8","resultStr":"{\"title\":\"Finding Homologs in Amino Acid Sequences Using Network BLAST Searches\",\"authors\":\"Istvan Ladunga\",\"doi\":\"10.1002/cpbi.34\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>BLAST, the Basic Local Alignment Search Tool, is used more frequently than any other biosequence database search program. We show how to run searches on the Web, and demonstrate how to increase performance by fine-tuning arguments for a specific research project. We offer guidance for interpreting results, statistical significance and biological relevance issues, and suggest complementary analyses. This unit covers both protein-to-protein (<i>blastp</i>) searches and translated searches (<i>blastx, tblastn, tfastx)</i>. <i>blastx</i> conceptually translates the query sequence and <i>tblastn</i> translates all nucleotide sequences in a database, while <i>tblastx</i> translates both the query and the database sequences into amino acid sequences. © 2017 by John Wiley & Sons, Inc.</p>\",\"PeriodicalId\":10958,\"journal\":{\"name\":\"Current protocols in bioinformatics\",\"volume\":\"59 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpbi.34\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current protocols in bioinformatics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpbi.34\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in bioinformatics","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpbi.34","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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