活体动物肾脏功能的多光子显微镜成像

Q1 Health Professions Current Protocols in Cytometry Pub Date : 2018-01-18 DOI:10.1002/cpcy.32
Kenneth W. Dunn, Timothy A. Sutton, Ruben M. Sandoval
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引用次数: 36

摘要

活体显微镜,活体动物显微镜,是一种强大的研究技术,它结合了培养细胞显微镜研究中发现的分辨率和灵敏度,以及完整动物背景下细胞的相关性和系统性影响。随着多光子荧光显微镜系统的发展,活体显微镜的功能得到了扩展,该系统能够在亚细胞分辨率下收集肾脏深处的光学切片,支持活体肾脏中肾小球、小管和脉管系统的结构和功能的高分辨率表征。将荧光探针施用于麻醉的、手术准备的动物,然后进行长达3小时的图像采集。图像通过高速网络传输到专门的计算机系统进行数字图像分析。这种通用方法可与荧光探针的不同组合用于评估肾小球渗透性、近端小管内吞作用、微血管流动、血管渗透性、线粒体功能和细胞凋亡/坏死等过程。©2018 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Live-Animal Imaging of Renal Function by Multiphoton Microscopy
Intravital microscopy, microscopy of living animals, is a powerful research technique that combines the resolution and sensitivity found in microscopic studies of cultured cells with the relevance and systemic influences of cells in the context of the intact animal. The power of intravital microscopy has recently been extended with the development of multiphoton fluorescence microscopy systems capable of collecting optical sections from deep within the kidney at subcellular resolution, supporting high‐resolution characterizations of the structure and function of glomeruli, tubules, and vasculature in the living kidney. Fluorescent probes are administered to an anesthetized, surgically prepared animal, followed by image acquisition for up to 3 hr. Images are transferred via a high‐speed network to specialized computer systems for digital image analysis. This general approach can be used with different combinations of fluorescent probes to evaluate processes such as glomerular permeability, proximal tubule endocytosis, microvascular flow, vascular permeability, mitochondrial function, and cellular apoptosis/necrosis. © 2018 by John Wiley & Sons, Inc.
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来源期刊
Current Protocols in Cytometry
Current Protocols in Cytometry Health Professions-Medical Laboratory Technology
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期刊介绍: Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.
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