[表达抗菌肽cecropin P1人工基因的凤尾莲植株的获得及特性]。

N S Zakharchenko, E B Rukavtsova, T V Shevchuk, O V Furs, S V Pigoleva, A A Lebedeva, I A Chulina, L K Baidakova, Ya I Bur'yanov
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引用次数: 0

摘要

已经获得了含有编码抗菌肽cecropin P1的人工CP1基因的羽状甘蓝植株。CP1基因在植物基因组中的存在已被PCR证实。MALDI质谱法和Western blotting法证实了转基因植物中Cecropin P1的合成。所获得的植物对细菌和真菌植物病原体具有高度抗性,其提取物对人类和动物病原体具有抗菌活性。已有研究表明,携带CP1基因的转基因植物可以被有益的结合微生物Methylovorus maays定植。
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[The obtainment and characteristics of Kalanchoe pinnata L. plants expressing the artificial gene of the cecropin P1 antimicrobial peptide].

Kalanchoe pinnata L. plants bearing an artificial CP1 gene encoding the cecropin P1 antimicrobial peptide have been obtained. The presence of the CP1 gene in the plant genome has been confirmed by PCR. Cecropin P1 synthesis in transgenic plants has been shown by MALDI mass spectrometry and Western blotting. The obtained plants have been highly resistant to bacterial and fungal phytopathogens, and their extracts have demonstrated antimicrobial activity towards human and animal pathogens. It has been shown that transgenic plants bearing the CP1 gene can be colonized by the beneficial associative microorganisms Methylovorus mays.

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