[瓦布贝母内生真菌赤霉病菌6WBY3产外多糖的理化性质及抗氧化活性]。

微生物学报 Pub Date : 2017-02-04
Feng Pan, Yunxin Yao, Xin Tang, Wei Wu
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引用次数: 0

摘要

目的:研究瓦布贝母内生真菌赤霉病菌6WBY3的胞外多糖(EPS)及其抗氧化活性。方法:采用有机溶剂脱脂、乙醇沉淀、大孔树脂脱色、蛋白酶联合sevag试剂脱蛋白、透析脱盐、deae -纤维素离子交换色谱分离等方法对菌株6WBY3培养基中EPS进行分离。然后,我们使用高效凝胶渗透色谱蒸发光散射检测器(HPGPC-ELSD),紫外(UV)光谱,扫描电镜(SEM),高效液相色谱(PMP- hplc)柱前衍生化,傅里叶变换红外(FT-IR)光谱和1H核磁共振(1H NMR)方法分析EPS馏分。我们用DPPH和ABTS自由基清除能力和铁离子螯合能力来评价EPS的抗氧化活性。结果:得到两种均相eps,分别为6WBY3EPS-3和6WBY3EPS-4,分子量(Mw)分别为17.41×106和8.84×105 Da。6WBY3EPS-3由甘露糖、葡萄糖和半乳糖组成,摩尔比为8.16:4.96:10.00;6WBY3EPS-4由甘露糖、鼠李糖、葡萄糖和半乳糖组成,摩尔比为8.08:1.71:6.32:10.00。SEM结果表明,6WBY3EPS-3为不规则多边体,6WBY3EPS-4为规则四边形体。FT-IR和1H NMR分析结果表明,6WBY3EPS-3为酸性多糖,含有丰富的半乳糖呋喃糖、甘露呋喃糖和少量α- d -葡萄糖吡喃糖,而6WBY3EPS-4主要含有吡喃糖环。这两种eps均具有α-(主)和β-糖苷构型的异构氢。此外,抗氧化活性结果表明,6WBY3EPS-3和6WBY3EPS-4具有较弱的DPPH自由基清除能力,中等的ABTS自由基清除能力和中等的铁离子螯合作用。结论:首次从内生真菌6WBY3中分离得到两个eps,并对其进行了研究。研究表明,从6WBY3中提取的EPS在医药和食品工业中具有作为抗氧化剂的应用潜力。此外,本研究也可为其他内生真菌开发EPS提供理论参考。
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[Physicochemical property and antioxidant activity of exopolysaccharide produced by endophytic fungal Fusarium redolens 6WBY3 isolated from Fritillaria unibracteata var. wabuensis].

Objective: To study the exopolysaccharide (EPS) of endophytic fungal Fusarium redolens 6WBY3 isolated from Fritillaria unibracteata var. wabuensis (FUW) including its antioxidant activities.

Methods: We isolated the EPS from the culture medium of strain 6WBY3 using the methods of degreasing with organic solvent, precipitation with ethanol, decoloration with macroporous resins, deproteinization with protease in combination with sevag reagent, desalination with dialysis and separation with DEAE-cellulose ion exchange chromatography. Then, we analyzed the EPS fractions using high-performance gel-permeation chromatography with evaporative light scattering detector (HPGPC-ELSD), ultra violet (UV) spectra, scanning electron microscope (SEM), PMP precolumn derivatization with high performance liquid chromatography (PMP-HPLC), fourier transform infrared (FT-IR) spectroscopy and 1H-nuclear magnetic resonance (1H NMR) methods. We evaluated the antioxidant activity of the EPS using DPPH and ABTS radical scavenging activities and iron ion chelating ability methods.

Results: We obtained two homogeneous EPSs, namely 6WBY3EPS-3 and 6WBY3EPS-4, with the molecular weight (Mw) of 17.41×106 and 8.84×105 Da, respectively. 6WBY3EPS-3 was composed of mannose, glucose and galactose in a molar ratio of 8.16:4.96:10.00, while 6WBY3EPS-4 was composed of mannose, rhamnose, glucose and galactose in a molar ratio of 8.08:1.71:6.32:10.00. The results of SEM showed that 6WBY3EPS-3 was the irregular multilateral body, and 6WBY3EPS-4 was rules of quadrilateral body. The results of FT-IR and 1H NMR analysis exhibited that 6WBY3EPS-3 was acidic polysaccharose with abundant galactofuranose, mannofuranose and a few α-D-glucopyranose, while 6WBY3EPS-4 possessed pyranose ring mainly. Those two EPSs had anomeric hydrogen with α-(main) and β-glycosidic configuration. Furthermore, the results of antioxidant activity suggested that both 6WBY3EPS-3 and 6WBY3EPS-4 had a weak DPPH radical scavenging ability, a moderate ABTS radical scavenging activity and a moderate iron ion chelating effect.

Conclusion: The two EPSs from the endophytic fungus 6WBY3 were firstly obtained and investigated. Our investigation indicated that the EPS from 6WBY3 had the application potential as an antioxidant in medicine and food industries. In addition, this work can also provide theory reference for improving development of EPS from other endophytic fungi.

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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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