一种用于成年小鼠脑结构神经解剖定量的旁矢状面切片方法

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2018-06-26 DOI:10.1002/cpmo.48

Stephan C. Collins, Christel Wagner, Léo Gagliardi, Perrine F. Kretz, Marie-Christine Fischer, Pascal Kessler, Meghna Kannan, Binnaz Yalcin

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引用次数: 18

摘要

在这篇文章中，我们提出了一个标准化的方案，用于快速和强大的成年小鼠大脑神经解剖学表型，这是对先前发表在《小鼠生物学当前协议》上的文章(doi: 10.1002/cpmo.12)的补充。它的目的是提供一个实验管道内的学术研究设置从实验工作到数据分析。我们的分析集中在一个单一的副矢状面，覆盖了涉及高阶认知的大部分大脑区域，如皮层、海马体和小脑，总共有166个区域、长度和细胞水平的测量参数，而我们之前发表的冠状面屏幕只有78个参数。本文讨论了使用旁矢状面分析进行大规模神经解剖筛查的好处。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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A Method for Parasagittal Sectioning for Neuroanatomical Quantification of Brain Structures in the Adult Mouse

In this article, we present a standardized protocol for fast and robust neuroanatomical phenotyping of the adult mouse brain, which complements a previously published article (doi: 10.1002/cpmo.12) in Current Protocols in Mouse Biology. It is aimed at providing an experimental pipeline within an academic research setting from experimental work to data analysis. Our analysis focuses on one single parasagittal plane, covering the majority of brain regions involved in higher order cognitions such as the cortex, hippocampus, and cerebellum, for a total of 166 parameters of area, length, and cell-level measurements in contrast to 78 parameters in our previously published coronal screen. Benefits of using parasagittal analysis for large-scale neuroanatomic screens are discussed. © 2018 by John Wiley & Sons, Inc.

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.