{"title":"脑膜全支架的制备及流式细胞术表征神经细胞","authors":"Antoine Louveau, Anthony J. Filiano, Jonathan Kipnis","doi":"10.1002/cpim.50","DOIUrl":null,"url":null,"abstract":"<p>Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous immune cells. Soluble factors produced by these cells are capable of diffusing into the brain parenchyma and influencing the brain cells within the parenchyma, including neurons. In this unit, we describe two protocols: the analysis of the meningeal compartment of rodents and the use flow cytometry to study the cells of the brain parenchyma (particularly neurons). © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":10733,"journal":{"name":"Current Protocols in Immunology","volume":"121 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpim.50","citationCount":"41","resultStr":"{\"title\":\"Meningeal Whole Mount Preparation and Characterization of Neural Cells by Flow Cytometry\",\"authors\":\"Antoine Louveau, Anthony J. Filiano, Jonathan Kipnis\",\"doi\":\"10.1002/cpim.50\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous immune cells. Soluble factors produced by these cells are capable of diffusing into the brain parenchyma and influencing the brain cells within the parenchyma, including neurons. In this unit, we describe two protocols: the analysis of the meningeal compartment of rodents and the use flow cytometry to study the cells of the brain parenchyma (particularly neurons). © 2018 by John Wiley & Sons, Inc.</p>\",\"PeriodicalId\":10733,\"journal\":{\"name\":\"Current Protocols in Immunology\",\"volume\":\"121 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-04-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpim.50\",\"citationCount\":\"41\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpim.50\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Immunology and Microbiology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Immunology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpim.50","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
引用次数: 41
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