{"title":"RNAi筛选:秀丽隐杆线虫的自动高通量液体RNAi筛选","authors":"Sakthimala Jagadeesan, Abdul Hakkim","doi":"10.1002/cpmb.65","DOIUrl":null,"url":null,"abstract":"<p>RNAi is a powerful reverse genetics tool that has revolutionized genetic studies in model organisms. The bacteriovorous nematode <i>Caenorhabditis elegans</i> can be genetically manipulated by feeding it an <i>Escherichia coli</i> strain that expresses a double-stranded RNA (dsRNA) corresponding to a <i>C. elegans</i> gene, which leads to systemic silencing of the gene. This unit describes protocols for performing an automated high-throughput RNAi screen utilizing a full-genome <i>C. elegans</i> RNAi library. The protocols employ liquid-handling robotics and 96-well plates. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":10734,"journal":{"name":"Current Protocols in Molecular Biology","volume":"124 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmb.65","citationCount":"5","resultStr":"{\"title\":\"RNAi Screening: Automated High-Throughput Liquid RNAi Screening in Caenorhabditis elegans\",\"authors\":\"Sakthimala Jagadeesan, Abdul Hakkim\",\"doi\":\"10.1002/cpmb.65\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>RNAi is a powerful reverse genetics tool that has revolutionized genetic studies in model organisms. The bacteriovorous nematode <i>Caenorhabditis elegans</i> can be genetically manipulated by feeding it an <i>Escherichia coli</i> strain that expresses a double-stranded RNA (dsRNA) corresponding to a <i>C. elegans</i> gene, which leads to systemic silencing of the gene. This unit describes protocols for performing an automated high-throughput RNAi screen utilizing a full-genome <i>C. elegans</i> RNAi library. The protocols employ liquid-handling robotics and 96-well plates. © 2018 by John Wiley & Sons, Inc.</p>\",\"PeriodicalId\":10734,\"journal\":{\"name\":\"Current Protocols in Molecular Biology\",\"volume\":\"124 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpmb.65\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Molecular Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpmb.65\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmb.65","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 5
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