Yan-mei Yang, De-dong Huang, You-yuan Lu, Li Lin, Xiao-hui Ma, Tian-tian Zhu, Ling Jin
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引用次数: 0
摘要
目的:建立龙胆根与大黄龙胆根的UPLC指纹图谱，并评价指纹图谱的相似性，为龙胆根的质量控制提供理论依据。方法:采用ACQUITY UPLC~BEH c18色谱柱(50 mm × 2)分离。1毫米,1。7 μm)通过甲醇-0梯度洗脱。04%的水磷，流速为0。3 m L/min。检测波长为242 nm，柱温为30℃。采用中药色谱指纹图谱相似度评价系统(2004 A版)对指纹图谱进行分析。结果:从13批龙胆根样品中筛选出13个共有峰作为指纹图谱峰，相似度为0。976到0。997，与大叶龙胆根进行指纹适用性试验的相似度较高(0。998)用龙胆根。结论:该方法精密度高，可靠性好，可用于龙胆药材的质量控制。
Objective: To establish UPLC fingerprints of Gentiana officinalis root and Gentiana macrophylla root,and to evaluate the similarity of the fingerprints,in order to provide theoretical basis for the quality control of Gentiana officinalis root.
Methods: The separation was performed on an ACQUITY UPLC~BEH C18column( 50 mm × 2. 1 mm,1. 7 μm) through a gradient elution of methanol-0. 04% aqueous phosphoric at a flow rate of 0. 3 m L/min. The detection wavelength was 242 nm, and the column temperature was set at30 ℃. Similarity evaluation system for chromatographic fingerprint of TCM( Version 2004 A) was used to analyze the fingerprints.
Results: 13 common peaks were selected as the fingerprint peaks from 13 batches of Gentiana officinalis root samples, with the similarities between 0. 976 to 0. 997, and Gentiana macrophylla root used for fingerprint applicability test has high similarity( 0. 998) with Gentiana officinalis root.
Conclusion: This method with good precision and reliability should be used for the quality control of Gentiana officinalis root.
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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