药用真菌蛹虫草遗传多样性的SCoT分析

中药材 Pub Date : 2016-07-01

Ting Li, Bin Song, Min Li

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引用次数: 0

摘要

目的:分析27株蛹虫草药用真菌的遗传多样性。方法:采用算术平均数(UPGMA)和主坐标分析法(PCoA)对27株蛹虫草菌株的起始密码子靶向多态性(SCoT)进行遗传多样性分析。结果:基于8条信息丰富的引物，共得到59条条带，94条条带。33%是多态的。多态性条带平均为6条。875. 结果表明，蛹虫草具有丰富的遗传多样性。采用UPGMA法将27个菌株划分为6个类群，采用PCoA法将27个菌株划分为3个类群，说明蛹虫草样品的遗传多样性与其地理来源有关。结论:SCoT标记信息丰富，可用于蛹虫草药用真菌的多态性检测。

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[SCoT Analysis on Genetic Diversity of Medicinal Fungus Cordyceps militaris].

Objective: To analyze the genetic diversity of medicinal fungus of 27 strains of Cordyceps militaris.

Methods: Unweighted pair-group method with arithmetic means( UPGMA) and principal coordinate analysis( PCoA) were used to determine the genetic diversity of 27 Cordyceps militaris strains based on start codon targeted polymorphism( SCoT).

Results: Based on eight informative primers,a total of 59 bands were produced,and 94. 33% of them were polymorphic. The average of polymorphism bands was 6. 875. The results suggested that great genetic diversity existed in Cordyceps militaris. Basically,27 strains were separated into six groups using UPGMA method,while three groups when using PCoA method,which showed that genetic diversity of Cordyceps militaris samples were related to their geographical origins.

Conclusion: SCoT markers are informative and could be used to detect polymorphism for medicinal fungus of Cordyceps militaris strains.

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.