紫穗槐根的HPLC指纹图谱及含量测定

中药材 Pub Date : 2016-09-01

Yu-wei Zhao, Miao Yu, Min-lun Nan, Na Lv, Xue-ling Si, Yu-fang He

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引用次数: 0

摘要

目的:建立紫穗槐根中毛蕊花素-7- o -β- d -葡萄糖苷、芒柄花素、毛蕊花素、芒柄花素的HPLC指纹图谱，同时测定其含量。方法:色谱柱为Diamonsil C18(250 mm ×4)。6mm,5 μm)。流动相为乙腈(A)-水(B)(含0。2%磷酸)梯度洗脱，检测波长为260 nm。采用“色谱指纹相似度评价软件”(2004A版)对10批药材进行相似度评价，采用SPSS软件进行聚类分析。结果:建立了紫穗槐根的高效液相色谱指纹图谱，分离效果好，并测定了紫穗槐根的4种化学成分。在HPLC指纹图谱中发现了16个共有峰。他们之间的相似度超过。90.结论:该分析方法特点强，重复性好，方法简便，可有效地用于魔芋根药材的质量控制。

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[HPLC Fingerprint and Determination in the Root of Amorpha fruticosa].

Objective: To establish HPLC fingerprint in the root of Amorpha fruticosa, and simultaneously to determine the content of calycosin-7-O-β-D-glucopyranoside, ononin, calycosin, formononetin.

Methods: The analytical column was Diamonsil C18( 250 mm ×4. 6 mm,5 μm). The mobile phase was acetonitrile( A)-water( B)( containing 0. 2% phosphoric acid) in gradient elution, and the detection wavelength was set at 260 nm. "Chromatographic fingerprint similarity evaluation software "version( 2004A) was used to evaluate similarity for the ten batches medicinal materials,and SPSS software was used for cluster analysis.

Results: The HPLC fingerprint of the root of Amorpha fruticosa was established with good separation, and four chemical compositions were determinated. 16 common peaks were defined in the HPLC fingerprint among the 10 batches of the root of Amorpa fruticosa. The similarity among them was more than0. 90.

Conclusion: This analytical method has strong features,with a good repeatability and the method is simple, which can be used efficiently in the quality control in the root of Amorpha fruticosa.

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.