[4个不同品种菊花中4种黄酮类化合物的比较研究]。

中药材 Pub Date : 2016-09-01

Han-zhen Liu, Ya-dong Shi, Hao Yu, Xin Xiao, Bin-bin Mao, Yan-xi Fang, Guo-liang Zhou

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引用次数: 0

摘要

目的:建立槲皮素、木犀草素、芹菜素、金合欢素的高相液相色谱含量测定方法，并比较4个不同品种菊花中槲皮素、木犀草素、芹菜素、金合欢素的含量差异。方法:采用超高效液相色谱法，色谱柱为Waters ACQUITYUPLC;色谱柱为BEH C18(50 mm ×2)。1毫米,1。7 μm)，流动相为0。1%磷酸溶液-甲醇梯度洗脱，流速为0。2 m L/min，检测波长为320 nm;柱温25℃;进样量为1 μL。结果:在0。0027年0。0135mg /m L(r1= 0。槲皮素浓度内峰面积呈良好的线性关系。在0的范围内。0032年0。0160 mg/m L(r2= 0。9963)木犀草素浓度内峰面积呈良好的线性关系。在0的范围内。0029年0。0145 mg/m L(r3= 0。在质量浓度和峰面积上，芹菜素有显著的差异。在0的范围内。0029年0。0145 mg/m L(r4= 0。9963)内阿曲素浓度与峰面积呈良好的线性关系。结论:该方法可测定菊花中槲皮素、木犀草素、芹菜素、金合欢素的含量。

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[Comparative Study on the Four Kinds of Flavonoids from Four Different Varieties of Dendranthema morifolium].

Objective: To establish a high phase liquid chromatography method of the content in quercetin,luteolin,apigenin,acacetin,and to compare the difference of content from four different varieties of Dendranthema morifolium in simultaneously.

Methods: The UPLC methods were adopted,and the chromatographic column was Waters ACQUITYUPLC; the column was BEH C18（ 50 mm ×2. 1 mm,1. 7 μm）,the mobile phase was 0. 1% phosphoric acid solution-methanol in gradient elution,the flow rate was 0. 2 m L/min;and the detection wavelength was set at 320 nm; the column temperature 25 ℃; and the sample quantity was 1 μL.

Results: In the range of 0. 0027 0. 0135 mg/m L（ r1= 0. 9962） concentration within quercetin in a good linear relationship between peak area. In the range of0. 0032 0. 0160 mg/m L（ r2= 0. 9963） concentration within luteolin in a good linear relationship between peak area. In the range of0. 0029 0. 0145 mg/m L（ r3= 0. 9964） of apigenin in the mass concentration and the peak area. In the range of 0. 0029 0. 0145 mg/m L（ r4= 0. 9963） concentration within acacetin in a good linear relationship between peak area.

Conclusion: This method can be determined daisy quercetin,luteolin,apigenin,acacetin content in Dendranthema morifolium.

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来源期刊

中药材

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.