Sirt1通过降低组蛋白3K9的乙酰化来保护神经干细胞免于凋亡。

IF 1.7 Q4 CELL BIOLOGY Stem Cells and Cloning-Advances and Applications Pub Date : 2018-09-07 eCollection Date: 2018-01-01 DOI:10.2147/SCCAA.S173852

Chongdong Jian, Cuihua Zou, Ning Xu, Guoying Chen, Donghua Zou

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引用次数: 3

摘要

目的:探讨Sirt1在神经干细胞(NSCs)凋亡保护中的作用及其机制。材料和方法:转染大鼠NSCs过表达Sirt1。观察Sirt1过表达对喜树碱诱导的NSCs凋亡的影响。Western blotting检测Sirt1、cleaved caspase-3和乙酰化组蛋白3K9的表达。结果:NSCs中Sirt1的过表达降低了caspase-3的裂解和组蛋白3K9的乙酰化。结论:Sirt1可能通过降低K9上组蛋白3的乙酰化而保护NSCs免于凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9.

Objective: To explore the role and mechanism of Sirt1 in protecting neural stem cells (NSCs) from apoptosis.

Materials and methods: Transfection was used to overexpress Sirt1 in rat NSCs. The effect of Sirt1 overexpression on camptothecin-induced apoptosis of NSCs was evaluated. Western blotting was used to examine the expression of Sirt1, cleaved caspase-3, and acetylated histone 3K9.

Results: Overexpression of Sirt1 in NSCs decreased the cleavage of caspase-3 and acetylation of histone 3K9.

Conclusion: Sirt1 may protect NSCs from apoptosis by decreasing the acetylation of histone 3 on K9.

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