[未染色切片可用于免疫染色多长时间?-抗原性保存时间的研究[j]。

Shinichi Inoue, Yoshinori Matsuki, Masaya Mori
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引用次数: 0

摘要

众所周知,当载玻片在室温下保存时,未染色的切片放在载玻片上的抗原性降低。据报道,有几种方法可以防止抗原性降低,例如在4°C的黑暗处保存,用石蜡或薄片覆盖切片,并在-80°C的深度冰箱中保存。本研究的目的是确定未染色切片可以在冰箱中保存多长时间(即1周,2周,1个月和2个月)。每个切片染色良好,与第0天切片相同,表达CD3、CD20、bcl-2、bcl-6、ER、PgR、HER2、Ki-67、嗜铬粒蛋白a、突触素、CK5、CK7、CK20和TTF-1。总之,保存至少2个月后,未染色的切片可用于免疫染色,制备几个对照切片并将其保存在冰箱中有助于减少对照块的损失。(最初的)。
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[How Long Are Unstained Sections Available for Immunostaining? -A Study of Antigenicity Preservation Time-].

It is well known that the antigenicity of unstained sections put on a slide glass decreases when the slides are stored at room temperature. Several methods have been reported to prevent reduced antigenicity, such as storing in a dark place at 4°C, covering sections with paraffin or a sheet, and storing in a deep freezer at -80°C. The aim of the present study is to determine how long unstained sections can be stored in a refriger- ator (ie, 1 week, 2 weeks, 1 month and 2 months). Each section was well stained and equal to the day 0 section for CD3, CD20, bcl-2, bcl-6, ER, PgR, HER2, Ki-67, chromogranin-A, synaptophysin, CK5, CK7, CK20, and TTF-1. In conclusion, unstained sections can be used for immunostaining after at least 2 months of storage, and preparing several control sections and storing them in a refrigerator is useful for minimizing control block loss. [Original].

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