{"title":"脑gabaar偶联Cl-/HCO3-活性的制备和测定及其在水生毒性综合评价中的应用","authors":"Sergey A. Menzikov","doi":"10.1002/cptx.70","DOIUrl":null,"url":null,"abstract":"<p>The wide use of aromatic hydrocarbons in various industries is having a negative effect on the environment and human health. Therefore, a key focus of current toxicology is the development and use of protein reporters with high sensitivity to various aromatic hydrocarbons (including phenolics and drugs). One molecular target for a wide range of pharmacology drugs and aromatic hydrocarbons (including phenol) is the neuronal GABA<sub>A</sub>R-coupled Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup>-ATPase. In this study, we present a protocol for isolation of the membrane-bound Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup>-ATPase from neuronal cells of animal brain. We then describe an uncomplicated <i>in vitro</i> method for measuring this ATPase activity for assessment of toxicity after interaction of this protein with an aquatic sample. This assay offers new avenues for using the Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup>-ATPase as a biomarker of water toxicity. This biotest is efficient, requires very little of the enzyme, and retains its sensitivity at low levels of various compounds. © 2019 by John Wiley & Sons, Inc.</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"80 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.70","citationCount":"0","resultStr":"{\"title\":\"Preparation and Measuring of Brain GABAAR-coupled Cl-/HCO3-- Activity for Integral Assessment of Aquatic Toxicity\",\"authors\":\"Sergey A. Menzikov\",\"doi\":\"10.1002/cptx.70\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The wide use of aromatic hydrocarbons in various industries is having a negative effect on the environment and human health. Therefore, a key focus of current toxicology is the development and use of protein reporters with high sensitivity to various aromatic hydrocarbons (including phenolics and drugs). One molecular target for a wide range of pharmacology drugs and aromatic hydrocarbons (including phenol) is the neuronal GABA<sub>A</sub>R-coupled Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup>-ATPase. In this study, we present a protocol for isolation of the membrane-bound Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup>-ATPase from neuronal cells of animal brain. We then describe an uncomplicated <i>in vitro</i> method for measuring this ATPase activity for assessment of toxicity after interaction of this protein with an aquatic sample. This assay offers new avenues for using the Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup>-ATPase as a biomarker of water toxicity. This biotest is efficient, requires very little of the enzyme, and retains its sensitivity at low levels of various compounds. © 2019 by John Wiley & Sons, Inc.</p>\",\"PeriodicalId\":72743,\"journal\":{\"name\":\"Current protocols in toxicology\",\"volume\":\"80 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cptx.70\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current protocols in toxicology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cptx.70\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.70","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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