磁性细胞分选用于体内和体外星形胶质细胞、神经元和小胶质细胞分析

Q2 Neuroscience Current Protocols in Neuroscience Pub Date : 2019-06-05 DOI:10.1002/cpns.71

Leanne M. Holt, S. Tristan Stoyanof, Michelle L. Olsen

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引用次数: 44

摘要

对评估中枢神经系统中单个细胞群体的兴趣促使了几种能够富集单细胞群体的技术的发展。在此，我们详细介绍了一种相对廉价的方法，利用结合细胞类型特异性抗体的磁珠从混合匀浆中特异性分离神经元、星形胶质细胞和小胶质细胞。我们已经用这种技术分离了发育过程中的星形胶质细胞和成年后期的星形胶质细胞。最后，我们详细介绍了该技术在星形胶质细胞和星形胶质细胞/神经元共培养范例中的应用。©2019 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Magnetic Cell Sorting for In Vivo and In Vitro Astrocyte, Neuron, and Microglia Analysis

Interest in evaluating individual cellular populations in the central nervous system has prompted the development of several techniques enabling the enrichment of single‐cell populations. Herein we detail a relatively inexpensive method to specifically isolate neurons, astrocytes, and microglia from a mixed homogenate utilizing magnetic beads conjugated to cell‐type specific antibodies. We have used this technique to isolate astrocytes across development and into late adulthood. Finally, we detail the utilization of this technique in novel astrocyte and astrocyte/neuron co‐culture paradigms. © 2019 by John Wiley & Sons, Inc.

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Current Protocols in Neuroscience Neuroscience-Neuroscience (all)

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期刊介绍： Current Protocols in Neuroscience is a one-stop resource for finding and adapting the best models and methods for all types of neuroscience experiments. Updated every three months in all formats, CPNS is constantly evolving to keep pace with the very latest discoveries and developments. A year of these quarterly updates is included in the initial CPNS purchase price.