趋磁细菌分离方法的设计与应用

IF 1.4 4区地球科学 Q3 GEOCHEMISTRY & GEOPHYSICS Acta Geochimica Pub Date : 2007-08-01 DOI:10.1007/s11631-007-0252-y

Xiao Zhijie, Lian Bin, Chen Jun, H. Henry Teng

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引用次数: 7

摘要

设计了一种简单的装置，根据趋磁细菌的趋磁性从土壤或沉积物中有效分离趋磁细菌。通过样品培养、MTB采集、分离、纯化和鉴定等一系列过程，从样品中成功分离出多株细菌。通过透射电子显微镜（TEM）和能量色散X射线分析（EDXA）证实这些细菌是趋磁细菌。根据16SrDNA序列构建系统发育树，推断出分离的磁性菌株与一些已知的趋磁细菌之间的系统发育关系。该装置已被证明具有价格低廉、组装简单、易于操作和高效分离新型趋磁细菌的优点。研究表明，采用自制装置采集MTB与平板菌落分离相结合的方法，可以有效地纯化和分离趋磁细菌。

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Design and application of the method for isolating magnetotactic bacteria

A simple apparatus was designed to effectively isolate magnetotactic bacteria from soils or sediments based on their magnetotaxis. Through a series of processes including sample incubation, MTB harvesting, isolation, purification and identification, several strains of bacteria were isolated from the samples successfully. By Transmission Electron Microscopy (TEM) and Energy-Dispersive X-ray Analysis (EDXA), these bacteria were certificated to be magnetotactic bacteria. The phylogenetic relationship between the isolated magnetic strains and some known magnetotactic bacteria was inferred by the construction of phylogenetic tree based on 16SrDNA sequences. This apparatus has been proven to have the advantages of being inexpensive, simple to assemble, easy to perform and highly efficient to isolate novel magnetotactic bacteria. The research indicated that the combined approach of harvesting MTB by home-made apparatus and the method of plate colony isolation could purify and isolate magnetotactic bacteria effectively.

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来源期刊

Acta Geochimica GEOCHEMISTRY & GEOPHYSICS-

CiteScore

2.80

自引率

6.20%

发文量

1134

期刊介绍： Acta Geochimica serves as the international forum for essential research on geochemistry, the science that uses the tools and principles of chemistry to explain the mechanisms behind major geological systems such as the Earth‘s crust, its oceans and the entire Solar System, as well as a number of processes including mantle convection, the formation of planets and the origins of granite and basalt. The journal focuses on, but is not limited to the following aspects: • Cosmochemistry • Mantle Geochemistry • Ore-deposit Geochemistry • Organic Geochemistry • Environmental Geochemistry • Computational Geochemistry • Isotope Geochemistry • NanoGeochemistry All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Acta Geochimica publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of geochemistry.