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{"title":"费氏弧菌:实验室培养、储存和常见表型分析","authors":"David G. Christensen, Karen L. Visick","doi":"10.1002/cpmc.103","DOIUrl":null,"url":null,"abstract":"<p><i>Vibrio fischeri</i> is a nonpathogenic organism related to pathogenic <i>Vibrio</i> species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of <i>V. fischeri</i>, as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of <i>V. fischeri</i>. © 2020 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Growth of <i>V. fischeri</i> from frozen stocks</p><p><b>Basic Protocol 2</b>: Growth of <i>V. fischeri</i> in rich, undefined liquid medium</p><p><b>Alternate Protocol 1</b>: Growth of <i>V. fischeri</i> in minimal medium</p><p><b>Basic Protocol 3</b>: Storage of <i>V. fischeri</i> in frozen stocks</p><p><b>Basic Protocol 4</b>: Biofilm assay on solid agar</p><p><b>Alternate Protocol 2</b>: Biofilm assay in shaking liquid culture</p><p><b>Alternate Protocol 3</b>: Biofilm assay in static liquid culture</p><p><b>Basic Protocol 5</b>: Motility assay</p><p><b>Basic Protocol 6</b>: Luminescence assay</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.103","citationCount":"13","resultStr":"{\"title\":\"Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays\",\"authors\":\"David G. Christensen, Karen L. Visick\",\"doi\":\"10.1002/cpmc.103\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Vibrio fischeri</i> is a nonpathogenic organism related to pathogenic <i>Vibrio</i> species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of <i>V. fischeri</i>, as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of <i>V. fischeri</i>. © 2020 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Growth of <i>V. fischeri</i> from frozen stocks</p><p><b>Basic Protocol 2</b>: Growth of <i>V. fischeri</i> in rich, undefined liquid medium</p><p><b>Alternate Protocol 1</b>: Growth of <i>V. fischeri</i> in minimal medium</p><p><b>Basic Protocol 3</b>: Storage of <i>V. fischeri</i> in frozen stocks</p><p><b>Basic Protocol 4</b>: Biofilm assay on solid agar</p><p><b>Alternate Protocol 2</b>: Biofilm assay in shaking liquid culture</p><p><b>Alternate Protocol 3</b>: Biofilm assay in static liquid culture</p><p><b>Basic Protocol 5</b>: Motility assay</p><p><b>Basic Protocol 6</b>: Luminescence assay</p>\",\"PeriodicalId\":39967,\"journal\":{\"name\":\"Current Protocols in Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpmc.103\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.103\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.103","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
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Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays
Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. fischeri , as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of V. fischeri . © 2020 Wiley Periodicals LLC.
Basic Protocol 1 : Growth of V. fischeri from frozen stocks
Basic Protocol 2 : Growth of V. fischeri in rich, undefined liquid medium
Alternate Protocol 1 : Growth of V. fischeri in minimal medium
Basic Protocol 3 : Storage of V. fischeri in frozen stocks
Basic Protocol 4 : Biofilm assay on solid agar
Alternate Protocol 2 : Biofilm assay in shaking liquid culture
Alternate Protocol 3 : Biofilm assay in static liquid culture
Basic Protocol 5 : Motility assay
Basic Protocol 6 : Luminescence assay
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