利用秀丽隐杆线虫模型分析白喉棒状杆菌的毒力。

Yi-Wei Chen, Hung Ton-That
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引用次数: 3

摘要

白喉链杆菌是咽部白喉的主要病因,咽部白喉是一种呼吸道疾病,其特征是在感染部位形成假膜。虽然目前白喉支原体感染的暴发很少见,但耐多药白喉支原体菌株的出现是全世界最重要的公共卫生问题之一。尽管人们对白喉芽孢杆菌的研究已有一个多世纪,白喉毒素和白喉毛已被确定为主要的毒力因素,但对细菌定植和疾病发展的相关因素知之甚少。在这里,我们描述了秀丽隐杆线虫作为一种具有成本效益的、通用的感染模型来评估白喉芽孢杆菌的毒力。我们提供了详细的方案,线虫同步和评估线虫的生存和形成变形的肛门区域诱导白喉C.感染。这些方案将允许未来对白喉支原体的毒力因子进行高通量筛选,并提高我们对白喉支原体发病机制的认识。©2020 Wiley期刊有限责任公司基本方案1:线虫的同步基本方案2:白喉C.感染后线虫存活的测定基本方案3:细菌定定和变形肛门区域形成的测定。
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Corynebacterium diphtheriae Virulence Analyses Using a Caenorhabditis elegans Model.

Corynebacterium diphtheriae is the leading cause of pharyngeal diphtheria, a respiratory disease characterized by formation of a pseudomembrane at the site of infection. Although outbreaks of C. diphtheriae infections are rare nowadays, the emergence of multidrug-resistant C. diphtheriae strains is one of the most significant public health concerns worldwide. Although C. diphtheriae has been studied for more than a century and diphtheria toxin and pili have been identified as major virulence factors, little is known about factors involved in bacterial colonization and development of disease. Here, we describe the utilization of Caenorhabditis elegans as a cost-effective, versatile model of infection to evaluate C. diphtheriae virulence. We provide detailed protocols for nematode synchronization and for evaluation of nematode survival and formation of a deformed anal region induced by C. diphtheriae infection. These protocols will permit future high-throughput screenings of virulence factors in C. diphtheriae and advance our knowledge of C. diphtheriae pathogenesis. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Synchronization of nematodes Basic Protocol 2: Assay for nematode survival following C. diphtheriae infection Basic Protocol 3: Assays for bacterial colonization and formation of deformed anal region.

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来源期刊
Current Protocols in Microbiology
Current Protocols in Microbiology Immunology and Microbiology-Parasitology
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期刊介绍: Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.
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